Upgrading of organic waste: production of the copolymer poly-3-hydroxybutyrate-co-valerate by Ralstonia eutrophus with organic waste as sole carbon source |
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| Authors: | Klaassien J Ganzeveld Annechien van Hagen Martin H van Agteren Wim de Koning Anton J M Schoot Uiterkamp |
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| Institution: | a Department of Chemical Engineering, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands;b Center for Energy and Environmental Studies, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands;c Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands |
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| Abstract: | Two types of fermented organic waste (trade and industry waste and fruit and vegetable waste) were successfully used as a sole carbon source to produce poly-3-hydroxybutyrate-co-valerate (PHBV) by Ralstonia eutrophus (formerly Alcaligenes eutrophus) via oxygen limitation.The production of PHBV could be optimized by optimizing the oxygen transfer through the fermentor. Thereby, a peak concentration of 1.1 g PHBV per liter cell suspension, 40 w% of cell dry weight, was obtained at an aeration rate of 0.24 mol O2/h·kg biomass. The yield of PHBV on the fatty acid concentration in the organic waste was 0.16 g polymer/g volatile organic matter. The process obtained, compares well with the commercial production process of PHBV based on glucose. |
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| Keywords: | Organic waste Poly-3-hydroxybutyrate-co-valerate Ralstonia eutrophus Environment Process parameters |
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