Molecular characterization of the zoanthid genus <Emphasis Type="Italic">Isaurus</Emphasis> (Anthozoa: Hexacorallia) and associated zooxanthellae (<Emphasis Type="Italic">Symbiodinium</Emphasis> spp.) from Japan |
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Authors: | James Davis Reimer Shusuke Ono Junzo Tsukahara Fumihito Iwase |
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Institution: | (1) Research Program for Marine Biology and Ecology, Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima, Yokosuka, Kanagawa 237-0061, Japan;(2) Biological Institute on Kuroshio, 560 Nishidomari, Otsuki, Kochi 788-0333, Japan;(3) Department of Marine Science, Biology and Chemistry, Faculty of Science, University of the Ryukyus, Senbaru 1, Nishihara, Okinawa 903-0213, Japan;(4) Miyakonojo Higashi High School, Mimata, Miyazaki 889-1996, Japan;(5) Department of Developmental Biology, Faculty of Science, Kagoshima University, Korimoto 1-21-35, Kagoshima, Kagoshima 890-0065, Japan |
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Abstract: | The zoanthid genus Isaurus (Anthozoa: Hexacorallia) is known from both the Indo-Pacific and Atlantic Oceans, but phylogenetic studies examining Isaurus using molecular markers have not yet been conducted. Here, two genes of markers mitochondrial cytochrome oxidase subunit
I (COI) and mitochondrial 16S ribosomal DNA (mt 16S rDNA)] from Isaurus specimens collected from southern Japan (n = 19) and western Australia (n = 3) were sequenced in order to investigate the molecular phylogenetic position of Isaurus within the order Zoantharia and the family Zoanthidae. Additionally, obtained sequences and morphological data (polyp size,
mesentery numbers, mesogleal thickness) were utilized to examine Isaurus species diversity and morphological variation. By comparing our obtained sequences with the few previously acquired sequences
of genera Isaurus as well as with Zoanthus, Acrozoanthus (both family Zoanthidae), and Palythoa spp. (family Spenophidae) sequences, the phylogenetic position of Isaurus as sister to Zoanthus within the Family Zoanthidae was suggested. Based on genetic data, Isaurus is most closely related to the genus Zoanthus. Despite considerable morphological variation (in particular, polyp length, mesentery numbers, external coloration) between
collected Isaurus specimens, all specimens examined are apparently conspecific or very closely related based on molecular data and observed
morphological variation within colonies. Additionally, obtained internal transcribed spacer of ribosomal DNA (ITS-rDNA) sequences
from symbiotic zooxanthellae (Symbiodinium spp.) from all Isaurus specimens were shown to be subclade C1-related Symbiodinium.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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