| 铬革渣资源化处理研究Ⅸ──胶原蛋白分子量及分子量分布 |
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| 引用本文： | 蒋挺大,张春萍,甘友新,甘纯玑.铬革渣资源化处理研究Ⅸ──胶原蛋白分子量及分子量分布[J].环境科学,1994,15(2):11-14. |
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| 作者姓名： | 蒋挺大 张春萍 甘友新 甘纯玑 |
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| 作者单位： | 中国科学院生态环境研究中心，福建农学院分析测试中心 |
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| 摘 要： | 以含０．０２％叠氮钠的蒸馏水为流动相，宽分布Ｔ１０和Ｔ７０葡聚糖为标样，用水相凝胶色谱法（ＷＰＧＣ）测定了从铬革渣中按不同条件提取的胶原蛋白的分子量及分子量分布。在实验中设定的反应时间和介质的ｐＨ值范围内胶原蛋白的数均分子量（Ｍｎ）均低于４０００，分子量分布较宽（ｄ＝Ｍｗ／Ｍｎ＝３．１９－８．３５）。
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| 关 键 词： | 铬革渣 凝胶渗透色谱 胶原蛋白 |
| 修稿时间： | 1993/7/30 0:00:00 |
Reclamation Treatment of Chrome Leather Scraps IX. The Molecular Weight and molecular Weight Distribution of Collaged Protein |
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| Jiang Tingda.Reclamation Treatment of Chrome Leather Scraps IX. The Molecular Weight and molecular Weight Distribution of Collaged Protein[J].Chinese Journal of Environmental Science,1994,15(2):11-14. |
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| Authors: | Jiang Tingda |
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| Abstract: | The moleculat weight and its distribution of thecollagen protein were determined with a water-phasegel chromatography (WPGC). The collagen proteinwas seperated from chrome leather scraps underdifferent reaction conditions. In WPGC, distilledwater was used as solvent,containing 0. 02%NaN2,and dextran with a broad molecular weightdistribution as standard. In the designed reaction timeand PH value range, the number average molecularweight (Mn) of collagen Protein Was below 4000,and the molecular weight distribution proves to bewide (d=Mw/Mn= 3. 19-8. 35). |
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| Keywords: | chrome leather scrap molecularweight gel chromatography |
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