Aspergillus sclerotiorum lipolytic activity and its application in bioremediation of high-fat dairy wastewater environments |
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Authors: | de Moura Dickel Jaíne Daiane Carvalho Jéssyca Ketterine Silveira Maruhen Amir Datsch Menegotto dos Santos Paulo Rodrigues Maria Luiza Fernandes Fagundes-Klen Marcia Regina Rosa Carlos Augusto Johann Susana Buzanello Cleide Viviane da Silva de Lucca Rosemeire Aparecida de Oliveira Santos Ana Raquel da Rosa Mauricio Ferreira |
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Affiliation: | 1.Engineering and Exact Sciences Center, State University Western Paraná, Toledo, PR, Brazil ;2.Department of Engineering and Exact, Federal University of Paraná, Palotina, PR, Brazil ;3.Centre de Recherche du CHU de Québec – Université Laval, Axe Oncologie, Quebec, QC, G1V 4G2, Canada ;4.Centre de Recherche Sur Le Cancer de L’Université Laval, Quebec, QC, G1R 3S3, Canada ;5.Microbiology Department, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil ; |
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Abstract: | Oils and grease (O&G) have low affinity for water and represent a class of pollutants present in the dairy industry. Enzyme-mediated bioremediation using biocatalysts, such as lipases, has shown promising potential in biotechnology, as they are versatile catalysts with high enantioselectivity and regioselectivity and easy availability, being considered a clean technology (white biotechnology). Specially in the treatment of effluents from dairy industries, these enzymes are of particular importance as they specifically hydrolyze O&G. In this context, the objective of this work is to prospect filamentous fungi with the ability to synthesize lipases for application in a high-fat dairy wastewater environment. We identified and characterized the fungal species Aspergillus sclerotiorum as a good lipase producer. Specifically, we observed highest lipolytic activity (20.72 U g−1) after 96 h of fermentation using sunflower seed as substrate. The fungal solid fermented was used in the bioremediation in dairy effluent to reduce O&G. The experiment was done in kinetic from 24 to 168 h and reduced over 90% of the O&G present in the sample after 168 h. Collectively, our work demonstrated the efficiency and applicability of fungal fermented solids in bioremediation and how this process can contribute to a more sustainable wastewater pretreatment, reducing the generation of effluents produced by dairy industries. |
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