苯并[a]芘对鲫鱼生物标志物的影响研究

研究了苯并a]芘暴露对鲫鱼(Carassius auratus)的几种生物标志物的影响.在鲫鱼接受3个不同质量分数苯并a]芘(BaP)的腹腔注射14 d后,分析了其肝脏7-乙氧基-3-异吩噁唑酮-脱乙基酶(EROD)活性,以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)等抗氧化酶活性和红细胞核异常(NA)率变化.结果显示:w(BaP)为1 mg/kg处理组的EROD活性显著升高(P＜0.001).w(BaP)为0.1和1 mg/kg处理组SOD活性显著低于对照组(P＜0.001),在暴露结束时,w(BaP)为1 mg/h处理组CAT活性显著低于对照组(P＜0.01),而w(BaP)为0.1和1 mg/kg处理组GPx活性被显著抑制(P＜0.001).表明BaP对EROD活性有诱导作用,而对SOD,CAT和GPx等抗氧化酶活性的抑制作用明显.所有处理组NA率与对照组均无显著差异.因此,鲫鱼EROD,SOD,CAT和GPx等酶活性的改变适于作为生物标志物,可以用来显示暴露于多环芳烃有机污染物鱼类的生活状态. 

Assessment of Various Biomarkers in Carassius auratus Exposed to Benzo[a]pyrene

The effects of the typical polycyclic aromatic hydrocarbons(PAHs) exposure on biomarkers in Carassius auratus were investigated.Fish were intraperitoneally injected with 0.01,0.1 and 1 mg/kg benzo a] pyrene(BaP).Changes of hepatic ethoxyresurufin-o-deethylase(EROD) activities,hepatic antioxidantive enzymes(i.e.Catalase(CAT),Superoxide dismutase(SOD),Glutathione peroxidase(GPx)) activities and the erythrocytic nuclear abnormalities(NA) frequency were analyzed at 14 d post-exposure.EROD activity significantly increased in fish treated with 1 mg/kg(P<0.001),however significant inhibition of CAT activities(P<0.01) was observed in the same treatment group.Hepatic SOD and GPx activities were significantly inhibited(P<0.001) in all fish treated with BaP but 0.01 mg/kg injected.The erythrocytic NA frequency was kept constant in all treatment groups.The results indicated that the changes of detoxicant and antioxident enzymes activities might be used to evaluate the effects of PAHs on fish.