高效石油烃降解菌SKD-1的分离、筛选及其降解性能

从孤岛油田石油污染土壤中分离到一株高效石油降解菌，命名为SKD-1。该菌株菌落表面湿润光滑、边缘整齐、圆形、不透明、乳黄色，能够利用葡萄糖和淀粉作为其生长的碳源和能源，其最适生长环境为碱性（pH8-10），在分别以正十六烷烃和原油为惟一碳源，温度为30℃，摇床（180r／min）培养的条件下，菌株SKD-1的降解率分别为66．1％和36．9％。16SrRNA基因序列分析表明，菌株SKD-1与不动杆菌AcinetobactercalcoaceticusSY-1同源性达99％。结合菌株SKD．1菌落形态、理化性质以及系统发育分析，可以鉴定菌株SKD-1属于不动杆菌属（Acinetobactersp．），序列登录号为AB774229。

Isolation, purification of high-efficient hydrocarbon degrading bacteria SKD-1 and its degradation characteristics

A strain named SKD-1 was isolated from Gudao oil field. The colonial morphology of this bacte- ria is wet-smooth, round, opacity and cream-yellow. Glucose and starch could be utilized as the carbon and en- ergy source by this bacteria. The optimum growth environment for strain SKD-1 was alkalinity. Its degrading rate of hydrocarbon reached 66. 1% and 36.9% respectively under the culturing conditions of 30℃ with shaking （180 r/min） , 16 paraffin or crude oil as the sole carbon sources in the medium. 16S rRNA sequence analysis showed a high similarity of 99% with the most related strain Acinetobacter calcoaceticus （X81661） , based its colo- nial morphology, physiochemical properties and phylogenetic tree results, strain SKD-1 was regarded to be one member of Acinetobacter genus and named Acinetobacter SKD-1 （AB774229）.