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| 高效阿特拉津降解菌株DNSIO降解条件优化 | |
| 引用本文： | 张庆媛,葛世杰,姜昭,王志刚,张颖. 高效阿特拉津降解菌株DNSIO降解条件优化[J]. 环境污染治理技术与设备, 2013, 0(3): 1169-1174 |
| 作者姓名： | 张庆媛 葛世杰 姜昭 王志刚 张颖 |
| 作者单位： | 东北农业大学资源与环境学院,哈尔滨150030 |
| 基金项目： | 哈尔滨市科技创新人才研究专项基金（2012PFXXN013）;国家新世纪优秀人才计划项目（NCET-10-0145）;黑龙江省杰出青年学者科学基金（JC201006）;黑龙江省新世纪优秀人才计划项目（1155-NCET4）06）;“十二五”农村领域国家科技计划（2011BAD04802-1）;国家自然科学基金资助项目（30970525） |
| 摘 要： | 从长期施用阿特拉津的寒地黑土耕层（0～10cm）土壤中筛选到一株能以除草剂阿特拉津为氮源生长的降解菌株，结合16SrRNA序列分析结果，将该菌株命名为Arthrobacter sp．DNSl0。在接种量为10。CFU／mL的条件下，菌株DNSl0在24h内对100mg／L阿特拉津的降解率为99．41％。单因子实验结果表明，菌株DNSl0适宜生长和降解的条件范围是：温度25～35＇12，pH值5．0～8．0，培养液盐度0．1％～2％，对阿特拉津最大耐受浓度可达1200mg／L。正交实验法进一步表明，该菌株保持较好生长及降解能力的最优方案是温度30℃，pH值7．5，培养液盐度0．5％。影响其降解能力的环境因素的主次顺序依次是：温度〉盐度〉pH值。 |
| 关 键 词： | 阿特拉津（AT） 菌株DNSIO降解能力 环境因素 |
Optimization for degradation conditions of atrazine-degrading strain DNSIO |
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| Zhang Qingyuan Ge Shijie Jiang Zhao Wang Zhigang Zhang Ying. Optimization for degradation conditions of atrazine-degrading strain DNSIO[J]. Techniques and Equipment for Environmental Pollution Control, 2013, 0(3): 1169-1174 | |
| Authors: | Zhang Qingyuan Ge Shijie Jiang Zhao Wang Zhigang Zhang Ying |
| Affiliation: | Zhang Qingyuan Ge Shijie Jiang Zhao Wang Zhigang Zhang Ying (School of Resources ＆ Environment, Northeast Agricultural University, Harbin 150030, China) |
| Abstract: | An atrazine-degrading strain, which grew with atrazine as the nitrogen source, was isolated from the top of black soil （0 - 10 em） in cold area of long-term applying atrazine. The strain was designated as Arthrobacter sp. DNS10 according to the sequence of 16S rRNA. It is capable of removing 100 mg/L atrazine in medium about 99.41% within 24 h with 10s CFU/mL inoculation. The results of single factor analysis show the optimal conditions for growth and degradation ability of DNSIO. The range of temperature is 25 - 35℃ , pH is 5.0 -8.0, medium salinity is 0. 1% -2% and the maximum resistance concentration of atrazine is 1 200 mg/L. Furthermore, based on orthogonal experiments, the optimal combination scheme for the growth and degradation ability of DNS10 is that temperature is 30℃ , pH is 7.5 and medium salinity is 0.5%. The primary and secondary order of environmental factors that influence the degradation ability of DNS10 is that： temperature 〉 salinity 〉 pH |
| Keywords: | atrazine（AT） strain D NSIO degradation ability environmental factor |
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