| 一株高效硝化菌的鉴定及其特性 |
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| 引用本文： | 邓斌,郑佳佳,傅罗琴,张小平,梁权,沈文英,李卫芬.一株高效硝化菌的鉴定及其特性[J].环境污染治理技术与设备,2013(9):3635-3641. |
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| 作者姓名： | 邓斌 郑佳佳 傅罗琴 张小平 梁权 沈文英 李卫芬 |
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| 作者单位： | [1]浙江大学动物科学学院农业部动物营养与饲料重点实验室,杭州310058 [2]绍兴文理学院生命科学学院,绍兴312000 |
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| 基金项目： | 国家“973”重点基础研究发展规划项目（2009CB118705）; 公益性行业（农业）科研专项（201203083）; 国家“水体污染控制与治理”科技重大专项（2012ZX07101-012-03） |
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| 摘 要： | 本研究通过测定菌株ABT01在不同初始氮浓度、pH、C/N、温度和溶氧条件下对氨氮的去除效果,获得该菌株的最佳应用条件。实验结果表明,当初始氨氮浓度低于40 mg/L时,该菌株的氨氮去除率高达85%以上。该菌株最适脱氨氮条件均为：pH 5.0-7.0、C/N=5、35℃、摇床转速150 r/min（溶解氧5.1 mg/L）,氨氮去除率最高达96.8%。同时该菌株经16S rDNA测序、细胞壁脂肪酸组成等鉴定方法,确定ABT01为枯草芽孢杆菌（Bacillus subtilis）。研究表明,枯草芽孢杆菌ABT01具有较好的氨氮去除能力,对水产养殖水质调控有潜在的应用价值。
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| 关 键 词： | 养殖水体 氨氮 降解 枯草芽孢杆菌 |
Identification and characteristics of a high-efficient nitrification bacterium |
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| Deng Bin,Zheng Jiajia,Fu Luoqin,Zhang Xiaoping,Liang Quan,Shen Wenying,Li Weifen.Identification and characteristics of a high-efficient nitrification bacterium[J].Techniques and Equipment for Environmental Pollution Control,2013(9):3635-3641. |
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| Authors: | Deng Bin Zheng Jiajia Fu Luoqin Zhang Xiaoping Liang Quan Shen Wenying Li Weifen |
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| Institution: | 1. Key Laboratory of Animal Nutrition ＆ Feed Science, Ministry of Agriculture, College of Animal Science, Zhejiang University, Hangzhou 310058, China; 2. Department of Biology, Life Science Conege of Shaoxing University, Shaoxing 312000, China). |
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| Abstract: | In order to optimize the growth condition for the strain ABT01,we evaluated the ammonium removal efficiencies with different initial ammonium concentrations,pH,C / N,temperature and DO.The observed ammonium removal rate was more than 85% in 24 hours at an initial ammonium concentration below 40mg / L.The result showed that the best conditions for ammonium removal were as follows： pH 5.0 - 7.0,C / N = 5,35℃ and 150 r / min（DO is about 5.1 mg / L）.Under the conditions,the highest ammonium removal rate could reach up to 96.8%.Through 16 S rDNA sequencing and fatty acid composition analysis of cell wall,the strain was identified as Bacillus subtilis.The results indicated that ABT01 had a potential role in ammonium removal in aquaculture practice. |
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| Keywords: | aquaculture water ammonia-nitrogen degradation Bacillus subtilis |
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