Microbial degradation of 17β -estradiol and 17α -ethinylestradiol followed by a validated HPLC-DAD method |
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Authors: | Ana R Ribeiro Maria F Carvalho Carlos MM Afonso Maria E Tiritan Paula ML Castro |
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Institution: | 1. Centro de Investiga??o em Ciências da Saúde , Instituto Superior de Ciências da Saúde-Norte , Gandra, Portugal;2. Centro de Biotecnologia e Química Fina, Escola Superior de Biotecnologia , Universidade Católica Portuguesa , Porto, Portugal;3. Centro de Biotecnologia e Química Fina, Escola Superior de Biotecnologia , Universidade Católica Portuguesa , Porto, Portugal;4. Centro de Química Medicinal da Universidade de Porto , Faculdade de Farmácia da Universidade do Porto , Porto, Portugal;5. Centro de Química Medicinal da Universidade de Porto , Faculdade de Farmácia da Universidade do Porto , Porto, Portugal |
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Abstract: | This work aimed at studying the biodegradation of two estrogens, 17α -estradiol (E2) and 17β -ethinylestradiol (EE2), and their potential metabolism to estrone (E1) by microbial consortia. The biodegradation studies were followed by High Performance Liquid Chromatography–Diode Array Detector (HPLC–DAD) using a specifically developed and validated method. Biodegradation studies of the estrogens (E2 and EE2) were carried out with activated sludge (consortium A, CA) obtained from a Wastewater Treatment Plant (WWTP) and with a microbial consortium able to degrade recalcitrant compounds, namely fluorobenzene (consortium B, CB). E2 was more extensively degraded than EE2 by CA whereas CB was only able to degrade E2. The addition of acetate as a supplementary carbon source led to a faster biodegradation of E2 and EE2. E1 was detected as a metabolite only during the degradation of E2. The 16S rRNA gene sequence analyses of strains recovered from the degrading cultures revealed the presence of the genera Pseudomonas, Chryseobacterium and Alcaligenes. The genera Pseudomonas and Chryseobacterium were retrieved from cultures supplied with E2 and EE2, while the genus Alcaligenes was found in the presence of E2, suggesting that they might be involved in the degradation of these compounds. |
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Keywords: | Biodegradation endocrine disrupting chemicals 17β-estradiol 17α-ethinylestradiol estrone HPLC-DAD |
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