Detection of Listeria monocytogenes in ready-to-eat food by Step One real-time polymerase chain reaction |
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| Authors: | Jaroslav Pochop Miroslava Kačániová Lukáš Hleba L'Ubomír Lopasovský Alica Bobková Lucia Zeleňáková |
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| Affiliation: | 1. Department of Microbiology , Slovak University of Agriculture in Nitra , Nitra , Slovak Republic;2. Department of Food Hygiene and Safety , Slovak University of Agriculture in Nitra , Nitra , Slovak Republic |
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| Abstract: | The aim of this study was to follow contamination of ready-to-eat food with Listeria monocytogenes by using the Step One real time polymerase chain reaction (PCR). We used the PrepSEQ Rapid Spin Sample Preparation Kit for isolation of DNA and MicroSEQ® Listeria monocytogenes Detection Kit for the real-time PCR performance. In 30 samples of ready-to-eat milk and meat products without incubation we detected strains of Listeria monocytogenes in five samples (swabs). Internal positive control (IPC) was positive in all samples. Our results indicated that the real-time PCR assay developed in this study could sensitively detect Listeria monocytogenes in ready-to-eat food without incubation. |
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| Keywords: | Real-time PCR Listeria monocytogenes detection kit ready-to-eat food. |
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