14C‐labeled aflatoxin B1 prepared with yeastlike cultures of Aspergillus parasiticus |
| |
Authors: | Linda K Jackson Alex Ciegler |
| |
Institution: | Agricultural Research Service, U.S. Department of Agriculture , Northern Regional Research Laboratory , Peoria, Illinois, 61604 |
| |
Abstract: | Abstract A simple method was developed to produce 14C‐labeled aflatoxin B1 by using the yeastlike phase of Aspergillus parasiticus NRRL 2999. Yeastlike cultures resulted from absence of manganese in a synthetic medium. Sodium acetate‐1‐14C had a 0.22% average incorporation; sodium acetate‐1,2‐14C, 0.70%. The average yield of labeled B1 was 10 mg/500 ml medium with an average specific activity of either 63.3 mCi/mole (C‐l label) or 194.3 mCi/mole (C‐1, 2 label). |
| |
Keywords: | labeled aflatoxin mycotoxin Aspergillus |
|
|