Fenton (H2O2/Fe) reaction involved in Penicillium sp. culture for DDT [1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane)] degradation |
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| Authors: | Cecilia Izcapa-Treviño Octavio Loera Araceli Tomasini-Campocosio Fernando Esparza-García Juan A Salazar-Montoya María D Díaz-Cervantes |
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| Institution: | 1. Departament of Biotechnology and Bioengineering , Center of Research and Advanced Studies of the National Polytechnic Institute , México;2. Chemical Risk Subdirection, National Center for Disaster Prevention , Ministry of Interior , México;3. Departament of Biotechnology , Universidad Autónoma Metropolitana-Iztapalapa , México |
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| Abstract: | The purpose of this work was to demonstrate that a Fenton (H2O2/Fe) reaction was involved in DDT 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane)] degradation in a culture of Penicillium sp. spiked with FeSO4. A commercial DDT mixture (10% DDE 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene], 30% o,p-DDT and 60% of p,p′ -DDT) of 10 mg L? 1 was used. Hydrogen peroxide (H2O2), tartaric acid and oxalic acid were identified at 18 h in culture media, with and without added DDT; this correlated positively with lowering of pH from 5.8 to 2.7. Lower concentrations of oxalic acid and H2O2 (7.9 and 52.6 mg L? 1, respectively) occurred in media with DDT at 30 h, in comparison to that one without DDT mixture (27.9 and 65.3 mg L? 1, respectively), at this time there was maximum degradation (87.7, 91.7 and 94.2%) for DDE, o,p-DDT and p,p′-DDT, respectively. We propose that the degradation of the DDT mixture by Penicillium sp. was through a Fenton reaction (H2O2/Fe) under acidic conditions produced in situ during the fungal culture amended with FeSO4. |
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| Keywords: | DDT Fenton reaction Penicillium sp carboxylic acid tartaric acid oxalic acid biodegradation |
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