Toxicological evaluation of bacterial decolourised anaerobically treated distillery effluent with common duckweed (Lemna minor) |
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Authors: | Chandra Ram Srivastava Archana |
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Institution: | Environmental Microbiology Section, Industrial Toxicology Research Centre, Post Box No. 80, M.G. Marg, Lucknow-226 001, UP, India. |
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Abstract: | Duckweed (Lemna minor) a small vascular plant, grows rapidly, is sensitive to a wide variety of toxicants and is easy to culture. A method is described that measures duckweed frond growth, chlorophyll, protein and biomass content as indicator of growth inhibition. The physico-chemical analysis of anaerobically treated distillery effluent revealed high BOD (28,000 mg/l), COD (52,400 mg/l) and dark brown colour (180,000 Co. Pt.). This effluent showed high toxicity to Lemna minor after 96 h of exposure in laboratory condition. EC50 of the fronds for chlorophyll, protein and biomass was found to be 25%, however, the bacterial decolourised effluent showed reduction of BOD (87.50%), COD (84.50%) and colour (76%). Further the toxicity evaluation with Lemna minor showed toxicity reduction up to 63% for all tested parameters. The EC50 noted for chlorophyll, protein and biomass was 100% concentration of decolourised effluent. |
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