| 粪大肠菌群酶底物法在环境应急监测中的应用 |
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| 引用本文： | 汤琳,李备军,龚海燕,张锦平.粪大肠菌群酶底物法在环境应急监测中的应用[J].环境监测管理与技术,2013,25(6):56-59. |
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| 作者姓名： | 汤琳 李备军 龚海燕 张锦平 |
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| 作者单位： | 上海市环境监测中心;上海市环境监测中心;华东师范大学;上海市环境监测中心 |
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| 基金项目： | 环境保护部科技标准基金资助项目（2011-10） |
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| 摘 要： | 结合国外粪大肠菌群的酶底物检测方法，针对某次突发环境污染事件，用酶底物法和标准方法多管发酵法同步检测受污染地表水中的粪大肠菌群，讨论酶底物法在应急监测中检测粪大肠菌群的适用性。结果表明，两种方法的测定数据显著相关，没有统计学差异（ P＞0．05）。相对于多管发酵法，酶底物法特异性强，检测时间短，二次污染少，符合应急监测的要求。
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| 关 键 词： | 粪大肠菌群 酶底物法 多管发酵法 环境污染 应急监测 |
Applications of Enzyme Substrate Method for Fecal Coliform in Environmental Emergency Monitoring |
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| TANG Lin,LI Bei-jun,GONG Hai-yan,ZHANG Jin-ping.Applications of Enzyme Substrate Method for Fecal Coliform in Environmental Emergency Monitoring[J].The Administration and Technique of Environmental Monitoring,2013,25(6):56-59. |
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| Authors: | TANG Lin LI Bei-jun GONG Hai-yan ZHANG Jin-ping |
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| Institution: | 1. Shanghai Environmental Monitoring Center, Shanghai 200030, China; 2. East China Normal University, Shanghai 200062, China) |
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| Abstract: | Based on the enzyme substrate method for detecting fecal coliform bacteria abroad and an abrupt environmental accidents , this article used the enzyme substrate method and the standard method （ multi-tube fer-mentation method ） to detect fecal coliform in water , to discuss the applicability of fecal coli enzyme substrate method for emergency environmental monitoring .The results showed that the two methods have no statistical difference （P〉0.05）.Enzyme substrate method has better specificity , shorter detection time and less secondary pollution to multi-tube fermentation method , which can be popularized and standardized . |
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| Keywords: | Fecal coliform Enzyme substrate method Multi-tube fermentation method Environmental pollution Emergency monitoring |
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