祁连山七一冰川及融水中细菌多样性的研究

通过荧光显微镜测定细菌密度,利用分离培养和16S rDNA序列分析细菌种类多样性,对祁连山七一冰川雪及融水中的细菌数量和多样性进行了研究.结果表明,七一冰川雪及融水中微生物总数为103~105cells.mL-1,可培养细菌数为0~600cfu.mL-1.恢复出的22株不同种类的细菌隶属于Bacteroidetes、Actinobacteria、Firmicutes和a,β,γ-Proteobacteria共6个类群,其中Bacteroidetes占总类群的80%,为优势类群;Pedobacter和Pseudomonas属为优势菌,占可培养细菌数量的90%.与青藏高原、南北极冰雪中微生物的比较分析发现Pantoea、Providencia、Terrabacter及Aerococcus这4个属和Oxalobacteraceae细菌是七一冰川的地方种类,在其它低温环境中未被发现.

Bacterial Diversity Recovered from Qiyi Glacier and Runoff, Qilian Mts.

Abundance and species diversity of bacteria were investigated respectively by epifJuorescence microscope, the culture method and the analysis of 16S rDNA genes, with snow and runoff samples from Qiyi Glacier in the Qilian Mts. According to the results, the total microbial cells and bacterial CFU range from 10~3 to 10~5 cells·mL~(-1) and 0-600 cfu·mL~(-1), respectively. The 16S rDNA gene of 22 bacterial isolates recovered from snow and runoff samples belong to following groups: Bacteroidetes, Actinobacteria , Firmicutes and α ,β, γ- Proteobacteria . Based on their 16S rRNA sequences, Bacteroidetes forms the largest cluster in terms of abundance (80% of all isolates) and Pedobacter and Pseudomonas form the dominant genera in terms of abundance (90% of all isolates) . Compared with bacteria revealed from ice and snow in Qinghai-Tibet Plateau, South Pole and North Pole which have been reported, the bacteria belonging to Pantoea, Providencia, Terrabacter, Aerococcus and Oxalobacteraceae are especially exist in Qiyi Glacier as far as we know.