基于物质剂量效应曲线形状的联合作用分析

为探明能否从剂量效应曲线形状研究物质的联合作用,选择5种不同类型的标准物质,分别为十二烷基苯磺酸钠(SDBS)、氟硅唑、三氯异氰尿酸、ZnSO_4·7H_2O、CdCl_2·2.5H_2O,借助青海弧菌Q67(Vibrio-qinghaiensis sp.-Q67)微孔板生物毒性检测法和非线性毒性单位法(TU'模型)进行了标准物质及二元混合物的急性毒性检测及联合作用评价分析。二元混合物按照经典的等毒性法配置,选择EC_5、EC_(10)、EC_(15)、EC_(20)、EC_(25)、EC_(30)、EC_(35)、EC_(40)、EC_(45)、EC_(50)等浓度水平。利用最小二乘法进行单一物质剂量效应拟合获得各物质的剂量效应方程。结果表明:5种不同作用机理的标准物质按照剂量效应曲线类型分为3类;SDBS的剂量效应曲线趋势与其和Q67的作用过程相吻合;曲线类型相同的3组二元组合均表现为加和作用;不同曲线类型的7组二元组合中有5组产生了不同程度的协同或拮抗作用,产生联合作用的百分比高达71.4%,强度最高达53%;从物质的剂量效应曲线形态特征研究物质的联合作用具有可能,且工作量较小。

Preliminary study on the joint action based on the chemical dose-response curve shape

The present paper is inclined to make an exploration of the possibility of the joint action of chemicals in the shape of the dose response curves (abbreviated as DRCs).For the said purpose,5 different types of reference materials were chosen,they are,the sodium dodecyl benzene sulfonate (abbreviated as SDBS),the flusilazole,the trichloroisocyanuric acid (abbreviated as TCCA),ZnSO4 · 7H2O,in addition to CdCl2 · 2.5H2O.As a matter of fact,Some acute toxicity tests about standard substances and binary mixtures were carried out in accordance with the government standard with the microplate luminometer biological toxicity identification and detection by using the Vibrio-qinghaiensis sp.nov.Q67 as the indicative organism.And,then,the binary mixtures can be prepared in a classic empirical approach known as the equal toxicity solutions (ETS) at the concentration level sets as EC5,EC10,EC15,EC20,EC25,EC30,EC35,EC40,EC4 and EC50.What is more,the experimental data can be mathematically manipulated to obtain the DRCs for each kind of individual chemical through a kind of non-linear least square (NLLS) calculation.The results show that there actually.exist only 3 kinds of substances of 5 chemicals with different functions on Q67 according to the type of DRCs,whose trend proves consistent with its interaction with Q67.The 3 groups of binary mixtures with the same kind of DRCs also indicate additive functions with no exception.On the other hand,among the 7 groups of binary mixtures made up of chemicals with the different kinds of DRC,5 groups among them can produce different levels of synergistic or antagonistic actions.The percentage of binary mixtures that produce joint action accounts for as much as 71.4％,and the maximum intension of the joint action is up to 53％.This indicates that exploring the joint action of substances from the morphological characteristics of DRCs has the advantage of less work and needs to study the comprehensive advantages in the future work.Thus,it can be concluded that this present study implies to present a noval approach to disclosing the joint actions of chemicals on a theoretical basis both from the point of view of chemistry and that of mathematics.