固定化GEM/CAS串联工艺强化处理阿特拉津废水

考察了固定化基因工程菌强化处理(GEM)/传统活性污泥处理(CAS)串联工艺对阿特拉津废水的处理效果,水力停留时间(HRT)对处理效果的影响,基因工程菌的生长和流失情况.结果表明,当HRT为4～24h,阿特拉津初始浓度为20mg/L,以实际生活污水为碳源时,串联工艺均可以实现对高浓度高负荷的阿特拉津生物强化处理.水力停留时间为24h时,固定化细胞反应器(串联工艺A段)的处理效果最好,阿特拉津平均去除率为96.64%,出水浓度为0.56ms/L.水力停留时间为12、8和4h时,平均去除率分别为88.59%、89.79%、88.61%.反应器在以上4个HRT时, COD平均去除率分别为72.76%、64.59%、66.16%和65.84%. 在整个反应过程中,没有出现大量工程菌流失的现象,同时在固定化颗粒的表面以及浅层均观察到了大量工程菌菌体,固定化颗粒的表面还出现了生物膜和菌胶团,反应结束时,颗粒形态完好,强度满足本工艺条件下长期使用的需求.

Bioaugmentation with Immobilized Genetically Engineered Microorganism (GEM)/CAS Process for Treatment of Atrazine Wastewater

Bioaugmentation with immobilized genetically engineered microorganism (GEM)/ conventional activated sludge (CAS) process for treatment of atrazine wastewater was investigated and effects of hydraulic retention time (HRT) on treatment efficiency, activities of GEM were discussed. The results showed that when HRT was 4 ~ 24h, significant removal of atrazine was obtained in the process with bioaugmentation. When the HRT was 24h, the first stage of the process got best treatment effect: average atrazine removal rate was 96.64% and average atrazine concentration in effluent was 0.56mg/L. When HRT was changed to 12h, 8h or 4h, the removal rate was 88.59%, 89.79% and 88.61% separately. The average removal of COD in 4 runs was 72.76%, 64.59%, 66.16% and 65.84% separately. Significant loss of GEMs was not observed in this study but strong growth activities, biofilm and zoogloea on the surface of immobile particles were observed via scanning electron microscope (SEM). The mechanical stability of the particles remained unchanged after nearly two-month operation.