采气污水中硫酸盐还原菌选育方法及其生长特性研究

从陕北某含菌采气污水中分离一株硫酸盐还原菌的过程中,对三种培养基进行了筛选,对分离出的菌株进行鉴定、数量检测,同时分别用光学显微镜和扫描电镜进行了形态观察。结果表明:该菌株革兰氏染色阴性,芽孢染色阴性,表面不光滑,有鞭毛,作摇摆式运动;菌体呈杆状或弧状,在SRB测试瓶中呈阳性反应,确定该菌株属于脱硫弧菌属(Desulfovibrio)。在适宜条件下,分离得到的SRB在筛选出的培养基中6h后进入对数生长期,48h达到生长最高峰,含菌量为9.5×108个/mL。

Study on Selection Methods and Growth Characteristics of Sulfate Reducing Bacteria in Gas Recovery Sewage

In the process of isolating a strain of sulfate reducing bacteria(SRB)in gas recovery sewage in Shanbei,three kinds of medium were screened.The existence and the amount of SRB were detected in the medium.Meanwhile,the shape was observed by a optical microscope and a scanning electron microscope(SEM).The results showed that:the cell of strain was found to be Gram-negative,non-spore-forming,rough surface,and motile by a flagellum.The cell was rod or arc,and the strain showed positive reaction in the SRB test bottle.It was concluded that the strain belonged to Desulfovibrio.The strain reached the logarithmic phase after inoculation for 6h,and reached a peak after 48hunder optimal conditions.The quantity of SRB was 9.5×10 8 /mL.