全程自养脱氮系统中氨氧化菌关键种群的变迁

全程自养脱氮是在高铵氮低溶氧条件下完全由自养菌群作用而将水中氮素脱除的现象。特异性扩增16SrDNA上的氨氧化菌特异的CTO基因片段,建立克隆文库并进行系统发育学分析,考察全程自养脱氮系统菌群驯化及退化过程中氨氧化菌的结构组成和其中关键种群的变迁,结果表明:在驯化过程中Nitrosospira属细菌和Nitrosomonas oligotropha细菌逐渐被能够耐受高氨氮低溶氧的Nitrosomonas europaea细菌所代替,后者可能是全程自养脱氮系统氨氧化步骤的主要参与者,而氨氧化菌的菌群演变可能并不是后来系统退化的原因。

Changing of Dominant Species in Ammonia-oxidizing Population for Deammonification

In order to analyze ammonia-oxidizing population in deammonification sludge and investigate the changing of dominant species during the sludge acclimatization and degeneration,CTO stretch of AOB-specific 16S rDNA was amplified and three gene libraries derived from 3 stages were constructed. Results of gene sequences and phylogenetic analysis showed that during sludge acclimatization and degeneration, ammonia oxidizers such as Nitrosospira spp. and Nitrosomonas oligotropha were taken place with Nitrosomonas europaea which was often present in ammonium-rich condition. Results also suggested that the changing of ammonia-oxidizing population may not cause the degeneration of deammonification reactor.