复合反硝化菌群培养及生化性能研究

根据反硝化过程中存在的问题,筛选到一株具有反硝化能力的菌株,鉴定为施氏假单胞菌(Pseudomonas stutzeri),该菌株与生物活性炭污泥共培养,得到复合反硝化菌群,并对反硝化菌群的生化降解性能进行了研究。结果表明:该复合菌群污泥生物显微照片呈不规则颗粒状,SVI为38~55mL/g,VSS为2.0g/L,无丝状菌存在;具有明显的反硝化NO3--N,NO2--N能力,容积负荷可达0.9kgNO3--N(/m3·d);单独NO3--N,NO2--N反硝化曲线基本相似,NO3--N,NO2--N共存基质反硝化过程存在底物竞争关系,但不发生NO2--N抑制反硝化效应;在DO<0.5mg/L条件下,反硝化效果良好;复合菌群可以在pH为9.3条件下发生良好反硝化反应而不受到pH抑制。

Culture of Complex Denitrification Sludge and Its Denitrification Properties

With the purpose to solve the problems in the bio-denitrification process,a strain with denitrification ability was separated and identified as Pseudomonas stutzeri. Complex denitrification sludge was cultured by the coupling growth of the strain and bio-active carbon sludge,and the denitrification properties were studied. Results showed that the microphoto shape of the complex denitrification sludge was granular irregularly with no filamentous bacteria. SVI was 38~55mL/g and VSS was 2.0g/L. As for the different denitrification substrates such as NO3--N and NO2--N,the denitrification curves were similar. There was substrates competitive relationship between NO3--N and NO2--N when they coexisted in the same simulated wastewater,but no inhibitive effect occured by NO2--N. When DO was below 0.5mg/L,the denitrification effect was good,and in the high pH of 9.3,denitrification could take place well. The volume loading could get to 0.9kgNO3--N(/m·3d).