| 化学物质的Ah受体效应重组基因酵母检测法的优化 |
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| 引用本文： | 王伟,胡建英,顾炜旻,刘燕花. 化学物质的Ah受体效应重组基因酵母检测法的优化[J]. 环境科学, 2004, 25(4): 155-158 |
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| 作者姓名： | 王伟 胡建英 顾炜旻 刘燕花 |
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| 作者单位： | 北京大学环境学院,北京,100871;北京大学环境学院,北京,100871;北京大学环境学院,北京,100871;北京大学环境学院,北京,100871 |
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| 基金项目： | 国家杰出青年科学基金项目(49925103);国家863计划项目(2002AA601140-6) |
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| 摘 要： | 接有芳香烃受体(AhR)基因和芳香烃受体核转位子蛋白(ARNT)基因的酵母Saccharomyces cerevisiae菌株是测定化学物质的Ah受体效应的方法之一.本实验通过优化各个实验条件,发现用0.2%葡萄糖作前培养液培养菌24 h,用2%半乳糖作化学物质暴露时的培养液,暴露在玻璃管时,暴露时间从原来的18 h缩短到8 h.并用已经优化的方法测定了六氯苯和五氯苯的Ah受体效应,得出六氯苯和五氯苯相对于TCDD的毒性等当量值(TEF)分别为0.018629和0.000294.
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| 关 键 词： | 重组基因酵母检测法 Ah受体效应 六氯苯 五氯苯 |
| 文章编号： | 0250-3301(2004)04-0155-04 |
| 收稿时间： | 2003-10-08 |
| 修稿时间： | 2004-01-15 |
Modification and Application of Recombinant Yeast Bioassay for Measuring the AhR Ligand Activity |
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| WANG Wei,HU Jian-ying,GU Wei-min and LIU Yan-hua. Modification and Application of Recombinant Yeast Bioassay for Measuring the AhR Ligand Activity[J]. Chinese Journal of Environmental Science, 2004, 25(4): 155-158 |
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| Authors: | WANG Wei HU Jian-ying GU Wei-min LIU Yan-hua |
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| Affiliation: | College of Environmental Science, Peking University, Beijing 100871, China. |
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| Abstract: | A recombinant yeast bioassay, a yeast Saccharomyces cerevisiae, in which the human AhR and ARNT complex are coexpressed, is one of the methods to screen the active AhR agonist. In this study, the original agonist test was modified. The exposure time was reduced from 18 to 8 hours when experiment was under the following conditions: (1) the yeast was cultured in 0.2% glucose medium for 24 hours; (2) chemical exposure was carried in 2% galactose medium in glass tube. Finally, the AhR acitivity of hexachlorobenzene and pentachlorobenzene were assessed, and their toxicity equivalent factors were found to be 0.018629 and 0.000294, respectively. |
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| Keywords: | recombinant yeast bioassay AhR agonist hexachlorobenzene pentachlorobenzene |
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