Improvement of production of lipopeptide antibiotic iturin A using fish protein期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Improvement of production of lipopeptide antibiotic iturin A using fish protein

Authors:	Umme Salma Zohora Mohammad Shahedur Rahman Abdul Wahab Khan Masahiro Okanami Takashi Ano

Affiliation:	1. Proximo Biotech, 1-50 Newtown, Dinajpur 5200, Bangladesh;2. Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan;3. Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Jahangirnagar University, Savar 1342, Dhaka, Bangladesh;4. Agriculture Group, Alps Business Creation Co., Ltd., Kawasaki 210-0855, Japan;5. Department of Biotechnological Science, Faculty of Biology-Oriented Science and Technology, Kinki University, 930 Nishimitani, Kinokawa-city, Wakayama, 649-6493, Japan;1. Unité « Enzymes et Bioconversion », National School of Engineers of Sfax, Tunisia;2. Higher Institute of Biotechnology of Sfax, Tunisia;1. National Institute of Nutrition (Indian Council of Medical Research), Tarnaka, Hyderabad, Andhra Pradesh, India;2. Central Food Technological Research Institute (Council of Scientific and Industrial Research), Resource Centre, Hubshiguda, Hyderabad, Andhra Pradesh, India;3. School of Pharmacy, Anurag Group of Institutions, Venkatapur, Andhra Pradesh, India;1. Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg University Mainz, Germany;2. Institute for Medical Biostatistics, Epidemiology and Informatics, University Medical Center of the Johannes Gutenberg University Mainz, Germany;1. Laboratoire des Procédés Biologiques, Génie Enzymatique et Microbien-ProBioGEM, UPRES-EA 1026, Polytech’Lille/IUT A, Université Lille Nord de France-Sciences et Technologies, F-59655 Villeneuve d’Ascq Cedex, France;2. Axe GePEB, Institut Pascal, UMR 6602, Université Blaise Pascal, CNRS, Clermont Université, BP 10448, F-63000 Clermont-Ferrand, France;3. Unité de Biotechnologies et Bioprocédés, Université Libre de Bruxelles, Av. F.-D. Roosevelt 50, C.P. 165/61, B-1050 Bruxelles, Belgium;4. Unité de Chimie Biologique Industrielle, Gembloux Agro-BioTech, Université de Liège, B-5030 Gembloux, Belgium;1. Tianjin Key Laboratory of Indoor Air Environmental Quality Control, School of Environmental Science and Engineering, Tianjin University, 92 Weijin Road, Tianjin, 300072, China;2. School of Civil Engineering, Dalian University of Technology (DUT), 2 Linggong Road, Dalian, 116024, China

Abstract:	To enhance the production of lipopeptide antibiotic iturin A, nutrient contents of the culture mediums were investigated in both submerged and biofilm fermentations. As a carbon source maltose and as nitrogen source, fish protein was used. In submerged fermentation maltose uptake was found lower (12%) compared to biofilm fermentation (15%) that was associated with higher cellular growth in biofilm. However, requirement of nitrogen (fish protein) concentration was found similar in both submerged and biofilm fermentations. Production of iturin A in submerged fermentation with 12% maltose and 5% fish protein was 4450 mg/L, and in biofilm fermentation it was 5050 mg/L when 15% maltose and 5% fish protein was used.

Keywords:	iturin A lipopeptide antibiotic biofilm fermentation
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