Characterization of the e ective cellulose degrading strain CTL-6 |
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Authors: | Yucai Lv Xiaofen Wang Ning Li Xiaojuan Wang Masaharu Ishii Yasuo Igarashi and Zongjun Cui |
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Institution: | 1. Alan G. MacDiarmid Research Institute of Renewable Energy, China Three Gorges University, Yichang 443002, China.
E-mail: Lv yucai@hotmail.com
2. College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing,2. College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing 100193, China,1. Alan G. MacDiarmid Research Institute of Renewable Energy, China Three Gorges University, Yichang 443002, China.
E-mail: Lv yucai@hotmail.com,2. College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing 100193, China,3. Graduate School of Agricultural and Life Sciences, The University of Tokyo, I-I-I Yayoi, Bunkyo-Ku, Tokyo 113-8657, Japan,3. Graduate School of Agricultural and Life Sciences, The University of Tokyo, I-I-I Yayoi, Bunkyo-Ku, Tokyo 113-8657, Japan and 2. College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing 100193, China |
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Abstract: | An efficient cellulose degrading bacteria exists in the thermophilic wheat straw-degrading community, WDC2. However, this strain cannot be isolated and cultured using conventional separation techniques under strict anaerobic conditions. We successfully isolated a strain of effective cellulose degrading bacteria CTL-6 using a wash, heat shock, and solid-liquid alternating process. Analysis of its properties revealed that, although the community containing the strain CTL-6 grew under aerobic conditions, the purified strain CTL-6 only grew under anaerobic culture conditions. The strain CTL-6 had a striking capability of degrading cellulose (80.9% weight loss after 9 days of culture). The highest efficiency value of the endocellulase (CMCase activity) was 0.404 micromol/(min mL), cellulose degradation efficiency by CTL-6 was remarkably high at 50-65 degrees C with the highest degradation efficiency observed at 60 degrees C. The 16S rRNA gene sequence analysis indicated that the closest relative to strain CTL-6 belonged to the genus Clostridium thermocellum. Strain CTL-6 was capable of utilizing cellulose, cellobiose, and glucose. Strain CTL-6 also grew with Sorbitol as the sole carbon source, whereas C. thermocellum is unable to do so. |
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Keywords: | cellulose-degrading bacterium cellulose degradation capability anaerobic bacterium Clostridium |
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