黄地老虎NPV增效作用的研究

对黄地老虎核型多角体病毒（ＡｓＮＰＶ）的多角体蛋白和病毒粒子组分进行分离纯化，分别与棉铃虫核型多角体病毒（ＨａＮＰＶ）混合感染三龄棉铃虫幼虫，发现此二种组分均能够提高棉铃虫幼虫的死亡率，缩短半致死时间，降低半致死浓度。使用酶联免疫吸附测定（ＥＬＩＳＡ）技术检验ＡｓＮＰＶ多角体蛋白和病毒粒子与粘虫颗粒体病毒增效因子（ＰｓＧＶ－ＳＦ）和ＨａＮＰＶ多角体蛋白的同源性，发现ＡｓＮＰＶ多角体蛋白和病毒粒子与

STUDY ON SYNERGISTIC ACTION OF AGROTIS SEGETUM NUCLEAR POLYHEDROSIS VIRUS

Polyhedrinand virion, which were isolated and purified from Agrotis setegum nuclear polyhedrosisvirus (AsNPV), are administered to 3-instar Heliothis armigera larvae with Heliothisarmigera nuclear polyhedrosis virus (HaNPV). Both polyhedrin and virion could increasemortality of H. armigera larvae, shorten half mortality time and decrease half mortalityconcentration. Enzyme-Linked Immunosorbent Assay was used to study the homologies betweenpolyhedrin,virion and synergistic factor of Pseudaletia separata Granulosis Virus(PsGV-SF),polyhedrin of HaNPV. Poor homology was discovered between AsNPVpolyhedrin,virion and PsGV-SF. AsNPV polyhedrin shows high homology with HaNPV polyhedrin.Synergistic mechanism of AsNPV is discussed. Fig 6, Tab 1, Ref 9