| 碱性气体对鼠卵营养液M16和M2pH的影响 |
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| 引用本文： | 王洪敏,熊一力,刘光泽,贾彦征,潘菲.碱性气体对鼠卵营养液M16和M2pH的影响[J].环境污染与防治,2000,22(5):10-12. |
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| 作者姓名： | 王洪敏 熊一力 刘光泽 贾彦征 潘菲 |
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| 作者单位： | 全军传染病中心转基因工程研究室,广州,510602 |
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| 摘 要： | 为了考察原核显微注射法制备转基因小鼠过程中Ｍ１６和Ｍ２鼠卵营养液的ＰＨ的影响因素，测定了不同存放条件下的Ｍ２和Ｍ１６营养液的ＰＨ，结果发现，Ｍ２ 完全开放的空气环境中放置７ｈ，ＰＨ升高２．６％（Ｐ〈０．００１），在半封闭的实验环境中放置４ｄ，ＰＨ升高３．７％（Ｐ〈０．００１）；Ｍ１６在相对密闭的条件下放置７ｄ和１５ｄ，ＰＨ分别升高４．５％（Ｐ〈０．００１）和６．４％（Ｐ〈０．００１），在半封闭的实
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| 关 键 词： | 转基因动物 环境污染 碱性气体 鼠卵营养液 监测 |
Effects of alkaline gas on pH of M16 and M2 mouse eggs nutrient medium |
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| Wang Hongmin,Xiong Yili,Liu Guangze,et al..Effects of alkaline gas on pH of M16 and M2 mouse eggs nutrient medium[J].Environmental Pollution & Control,2000,22(5):10-12. |
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| Authors: | Wang Hongmin Xiong Yili Liu Guangze |
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| Institution: | Wang Hongmin,Xiong Yili,Liu Guangze,et al.Transgenic Engineering Research Lab,Infectious Disease Center of PLA,Guangzhou 510602 |
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| Abstract: | s:In order to examine the interfering factors in the course of generation of transgenic mice by pronuclear microrinjection,the pH of M2 and M16 were measured under different conditions.pH of M2 increased by 2 6%(p<0 001)when it was placed in the open air for 7 hours,3 7%(p<0 001)in the semi closed condition for 4 days.In the case of M16,pH rise of 4 5%(p<0 001)and 6 4%(p<0 001)were found in the relatively closed condition,17 0%(p<0 001)and 18 6%(p<0 001)when placed in the semi closed condition for 7 days and 15 days,respectively,pH increased by 20 7%(p<0 001)in the open air only for 7 hours;while pH of M16 kept relatively constant in the CO 2 incubator of 5 0% CO 2 and 95 0% of air for consecutive days.It was found that the pH change of M16 and M2 medium was caused by alkaline gas in the ambient air. |
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| Keywords: | Transgenic animal Environmental pollution M16 M2 pH |
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