| 一株高效溶藻放线菌的分子生物学鉴定 |
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| 引用本文： | 王慧玲,叶新,赵以军,程凯.一株高效溶藻放线菌的分子生物学鉴定[J].环境科学与技术,2009,32(6). |
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| 作者姓名： | 王慧玲 叶新 赵以军 程凯 |
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| 作者单位： | 华中师范大学城市水环境生态学湖北省重点实验室,湖北,武汉,430079 |
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| 基金项目： | 国家自然科学基金，湖北省科技攻关计划招标项目 |
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| 摘 要： | 实验室从土壤中分离筛选得到了一株具有高效溶藻能力的放线菌,命名为AN02。对这株具有高效溶藻效果能力的放线菌AN02分别进行了形态特征、培养特征及生理生化特性的分析,分析结果表明放线菌AN02菌株的上述几个特征与细黄链霉菌(Streptomyces microflavus)的高度一致,与此同时,聚类分析结果也同样表明,放线菌AN02菌株与细黄链霉菌(Streptomyces microflavus)的高度一致,后经过比对,发现二者的16SrDNA序列的同源性高达近100%,因此,可将溶藻放线菌AN02定名为细黄链霉菌AN02(Streptomyces microflavus AN02)。
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| 关 键 词： | 溶藻链霉菌AN02 分子学鉴定 16SrDNA序列 |
Molecular Biology Identification of Alga-lysing Actinomycete AN02 |
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| WANG Hui-ling,YE Xin,ZHAO Yi-jun,CHENG Kai.Molecular Biology Identification of Alga-lysing Actinomycete AN02[J].Environmental Science and Technology,2009,32(6). |
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| Authors: | WANG Hui-ling YE Xin ZHAO Yi-jun CHENG Kai |
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| Institution: | Hubei Key Laboratory of Urban Water Environmental Ecology;Central China Normal University;Wuhan 430079;China |
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| Abstract: | By analyzing the morphological,cultural,physiological and biochemical characteristics of the strain Actinomycete AN02 isolated from the soil by the laboratory with highly efficient alga -lysing ability,it was indicated that all these characteristics were highly accorded with those of Streptomyces microflavus.Homology analysis showed that its 16S rDNA sequence has the identity of 100% compared to Streptomyces microflavus.As a result,alga-lysing actinomycete AN02 could be named as Streptomyces microflavus AN0... |
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| Keywords: | alga-lysing Streptomyces AN02 molecular biology identification 16S rDNA sequence |
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