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Preparation of high resolution chromosomes from amniotic fluid cells |
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| Authors: | J J Hoo Heidi Jamro Sheila Schmutz C C Lin |
| Institution: | 1. Department of Pediatrics, University of Calgary, Health Sciences Centre, 3330 Hospital Drive, N. W., Calgary, Alberta T2N 4N1, Canada
Department of Pathology, University of Calgary, Health Sciences Centre, 3330 Hospital Drive, N. W., Calgary, Alberta T2N 4N1, Canada;2. Department of Pediatrics, University of Calgary, Health Sciences Centre, 3330 Hospital Drive, N. W., Calgary, Alberta T2N 4N1, Canada Department of Medical Biochemistry, University of Calgary, Health Sciences Centre, 3330 Hospital Drive, N. W., Calgary, Alberta T2N 4N1, Canada |
| Abstract: | A relatively simple method of obtaining high resolution chromosomes from amniotic fluid cells is described. The elongated chromosomes are achieved by adding ethidium bromide (5μg/ml) to the culture 4 1/2 hours before harvesting and the high resolution banding can be produced by usual banding procedures. |
| Keywords: | Amniotic fluid cells Ethidium bromide High resolution chromosomes Prenatal diagnosis |