Lack of sampling site variation in chorion villus biopsy as assessed by DNA,enzymatic, morphological and cytogenetical analyses |
| |
| Authors: | Meena Upadhyaya Bharat Jasani Elizabeth Little Peter S Harper Derek Rees Adrian Roberts |
| |
| Institution: | 1. Section of Medical Genetics, University of Wales College of Medicine, Heath Park, Cardiff, Wales, U.K.;2. Department of Pathology, University of Wales College of Medicine, Heath Park, Cardiff, Wales, U.K.;3. Cytogenetics Unit, University of Wales College of Medicine, Heath Park, Cardiff, Wales. U.K.;4. Department Obstetrics and Gynaecology, University of Wales College of Medicine, Heath Park, Cardiff Wales. U.K. |
| |
| Abstract: | A study of villus samples from eight random sites on five electively aborted chorion sacs was performed to determine any significant differences in yield, quality and composition of DNA, iduronate sulphate sulphatase activity and karyoptype status. The villi were also examined for their histological characteristics (e.g. stem, intermediate or terminal villi) and for HCG and BGP immuno-reactivities. The overall findings indicated no significant site to site variations in any of the parameters studied. It is therefore proposed that any villus should be equally suitable for prenatal diagnosis. |
| |
| Keywords: | Chorionic villus sampling Prenatal diagnosis DNA |
|
|
