碱处理污泥发酵产氢生物相特征

为了揭示碱处理污泥发酵产氢生物相的特征,分别将取自3个不同处理工艺污水厂的污泥进行碱处理,并分别在酸性(pH5)和碱性(pH11)的条件下进行发酵产氢.结果表明,虽然污泥来源不同,但经碱处理后溶出的可溶性有机质均以蛋白质居多,碳水化合物的量仅是蛋白质的15%～16%,在初始pH11的碱性条件下发酵产氢均可获得较高的产氢率,最大(以H2/TCOD计)可达31.9mL/g,但在初始pH5的酸性条件下产氢率不高,且伴有耗氢现象.用F338GC/R534细菌16SrDNA通用引物对发酵产氢结束时的生物相进行PCR-DGGE分析,不同污水厂污泥产氢的生物相差异显著.随着发酵的进行,生物相中优势菌群有增多的趋势,产氢反应过程中微生物菌群表现出更替消长的现象.

Characteristics of Microbial Community in Biohydrogen Production from Alkali Pretreated Sludge

In order to reveal the characteristics of microbial community in biohydrogen production from alkali pretreated sludge, the biohydrogen fermentation was conducted under acidic condition (pH 5) and alkali condition (pH 11) using alkali pretreated sludge from three wastewater treatment plants with different process, respectively. The results indicate that, although the sludge from different sources, protein is main component in the soluble organic matter released from the three sludge samples by the alkali pretreatment and carbohydrate is only 15%-16% of protein. A high hydrogen yield occurs at the condition of initial pH 11, a maximal hydrogen yield of up to 31.9 mL/g, while the hydrogen yield decreases and hydrogen consumption occurs at the condition of initial pH 5.The analysis using PCR-DGGE technique based on 16S rDNA sequences with the universal primers (F338GC and R534) show that the microbial community in the biohydrogen production process from various sludge structure is significant different, and the microbial population appear a phenomenon of turnover growth and decline and the amount of dominant microbial community present a increasing trend.