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| 镉对松散和紧密胞外聚合物类蛋白的荧光滴定 | |
| 引用本文： | 康福星,龙健,潘响亮,胡继伟,王倩.镉对松散和紧密胞外聚合物类蛋白的荧光滴定[J].环境科学学报,2009,29(2):360-366. |
| 作者姓名： | 康福星 龙健 潘响亮 胡继伟 王倩 |
| 作者单位： | 1. 贵州省山地环境信息系统与生态环境保护重点实验室,贵阳,550001;贵州师范大学,地理与环境科学学院,贵阳,550001 2. 中国科学院地球化学研究所环境国家重点实验室,贵阳,550001 3. 贵州师范大学,地理与环境科学学院,贵阳,550001 4. 贵州省山地环境信息系统与生态环境保护重点实验室,贵阳,550001 |
| 基金项目： | 贵州省自然科学基金，贵州师范大学研究生创新基金，国家高技术研究发展计划(863计划) |
| 摘 要： | 采用分子荧光技术和荧光滴定的方法解析了重金属cd与2种不同类型胞外聚合物类蛋白的结合机理;并通过2种胞外聚合物类蛋白与cd结合后荧光光谱的特征,进一步分析松散附着(Loosely Bound,LB)和紧密黏附(Tightly Bound.TB)2种胞外聚合物之间的结构差异.结果表明,在滴定的cd浓度低于1×10-mol·L-1、pH值为4的条件下,cd对LB具有显著的猝灭效应;结合Stern-Volmer分析,这种猝灭现象不仅与生色团的质子化效应有关.而且也和类蛋白与cd结合位点的变化有关.同时.滴定过程中LB均出现不同程度的红移现象,而TB却出现不同程度的蓝移.这说明,cd与2种类蛋白物质的结合机理方面具有显著差异.进一步三维光谱分析结果显示,在滴定前后LB中类蛋白荧光峰的结构与形态并未产生明显分化,由此说明,LB内荧光生色团性质和结构相对单一;而滴定后TB中肩峰的凸现说明.其类蛋白性质、结构与LB相比更具有多样性的特点.pH值为10的条件下,Stem-Volmer分析结果显示,LB和TB类蛋白荧光团的猝灭与2方面因素有关,一是与生成不产生荧光的EPS-Cd络合物有关,二是与分子之间的碰撞所导致的荧光猝灭有关. |
| 关 键 词： | 荧光滴定 类蛋白 猝灭 |
| 收稿时间： | 4/7/2008 9:49:19 AM |
| 修稿时间： | 7/23/2008 2:20:54 PM |
Fluorescence titration of cadmium with protein-like loosely and tightly bound extracellular polymeric substances |
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| KANG Fuxing,LONG Jian,PAN Xiangliang,HU Jiwei and WANG Qian.Fluorescence titration of cadmium with protein-like loosely and tightly bound extracellular polymeric substances[J].Acta Scientiae Circumstantiae,2009,29(2):360-366. | |
| Authors: | KANG Fuxing LONG Jian PAN Xiangliang HU Jiwei and WANG Qian |
| Institution: | KANG Fuxing1,2,LONG Jian1,PAN Xiangliang3,HU Jiwei2,WANG Qian11. Key Laboratory for Information System of Mountainous Areas , Protection of Ecological Environment of Guizhou Province,Guizhou Normal University,Guiyang 5500012. School of Geographic , Environmental Sciences,Guiyang 5500013. State Key Laboratory of Environmental Geochemistry,Institute of Geochemistry,Chinese Academy of Sciences,Guiyang 550001 |
| Abstract: | Molecular fluorescence technology and fluorescence titration methods were applied to elucidate the mechanism of association between the protein-like regions of two extracellular polymeric substances (EPS) and the heavy metal Cd. After combining the protein-like EPS with Cd, fluorescence spectra were used to further analyze the difference of configuration between the loosely bound extracellular (LB) and tightly bound extracellular polymeric substance (TB). An obvious quenching effect of LB triggered by Cd co... |
| Keywords: | LB TB |
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