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Molecular characterization of bacterial community in aerobic granular sludge stressed by pentachlorophenol
Authors:LIU He  LI Guangwei  LI Xiufen and CHEN Jian
Institution:1. Laboratory of Environmental Biotechnology, School of Bioteclmology, Jiangnan University, Wuxi 214122, China
2. Laboratory of Environmental Biotechnology, School of Bioteclmology, Jiangnan University, Wuxi 214122, China;Key Laboratory of lndustrial Biotechnology, Ministry of Education of China, Jiangnan University, Wuxi 214122, China
Abstract:To characterize the effects of pentachlorophenol (PCP) on the performance and microbial community of aerobic granular sludge in sequencing batch reactor (SBR), the web-based terminal restriction fragment length polymorphism (T-RFLP) and real-time PCR (RT- PCR) techniques were used to explore the bacterial community structure. When PCP increased from 0 to 50 mg/L, the COD removal rate changed little, while the ammonia removal rate dropped from 100% to 64.9%. The results of molecular characterization showed t...
Keywords:aerobic granular sludge  bacterial community  real-time PCR  terminal restriction fragment length polymorphism  sequencing batch reactor  peutachloropbenol(PCP)
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