Localization and biochemical characterization of pharyngeal protease in the polychaetous annelid Glycera convoluta |
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Authors: | C Michel J -M Imhoff |
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Institution: | 1. Département de Cytologie, Université de Parìs VI, Paris, France 2. Service de Chimie des Protéines, Institut Pasteur, Paris, France
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Abstract: | In Glycera convoluta Keferstein, pharyngeal protease is secreted by the glandular epithelial cells, as clearly demonstrated by microscopic observation of the digestion of a prestained gelatin film by fresh tissue sections of the organ. The enzyme, extracted by affinity chromatography on Sepharose-?-aminocaproïc-D-tryptophan, acts on substrates hydrolyzable by α-chymotrypsin such as N-benzoyl-L-tyrosine ethyl ester and carboxyl-propionyl-phenylalanine-p-nitroanilide, and can be considered as a chymotrypsin-like enzyme. |
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