Prototype design for a closed marine system employing quarternary water processing

A small 2, 271-1 (600-gallon) prototype recirculating marine water system was designed and evaluated (26 months). The system employed a quaternary (mechanical, algal, bacterial and chemical) series of processing units with the aim of achieving long-term seawater quality and constancy without water replacement. Total ammonia (0.05±0.005 ppm), un-ionized ammonia (0.0036±0.0003 ppm), nitrite (0.10±0.014 ppm), nitrate (24.6±2.0 ppm), phosphate (14.0±2.1 ppm), dissolved oxygen (6.5–7.2 mg/l) and pH (7.80 to 8.25) were maintained at acceptable levels for the entire period. The biomass load (1.82 kg/397 l) consisted of 4 nurse sharks which were fed ad-lib twice a week. Further, low levels of total bacteria (1.5×102 bacteria/ml) and coliforms [most probable number (MPN) index 17/dl] were present in the seawater. The system design is suggested for use in laboratory and mariculture installations.