基于SCGE的五氯酚对稀有鮈鲫DNA损伤的研究

采用单细胞凝胶电泳(SCGE)技术,研究了在不同的染毒时间内不同浓度的五氯酚(PCP)对稀有鮈鲫(Gobiocypris rarus)血细胞和肝脏细胞DNA的损伤.结果显示,各PCP染毒组细胞彗星尾部DNA含量 (%DNAT) 和彗星尾长(TL) 显著增加,与空白对照组比较,差异极显著(P0.926,说明在实验浓度范围内, 存在显著的浓度-效应关系.在同一浓度下,随着暴露时间的增加,处理组%DNAT和TL逐渐增加,存在显著的时间-效应关系.由于PCP可引起稀有鮈鲫血细胞和肝脏细胞DNA的严重损伤,因此稀有鮈鲫血细胞和肝脏细胞DNA的损伤可作为PCP遗传毒性的指示.

Alkaline single cell gel electrophoresis assay(SCGE) based study on DNA damage of Gobiocypris rarus induced by pentachlorophenol

By using the alkaline single cell gel-electrophoresis (SCGE), the DNA damage in blood cells and liver cells of Gobiocypris rarus caused by pentachlorophenol at different exposure time (lday, 4days, 7days) with different concentration of pentachlorophenol (40, 100, 160μg/L) was evaluated. Tail DNA percentage (%DNAT) and tail length (TL) of the three tested groups were significantly different from the control group (P< 0.05). Furthermore, the damage intensity of treated groups increased gradually with respect to the increasing of pentachlorophenol. The concentration-effect relationship was significant for the correlation coeffiencient was graeter than 0.926. Under the same concentration, the % DNAT and TL increased gradually with the time of exposure. The time-effect relationship was also significant. This study recommended that it could be a sensitive monitor of aquatic pollution to use the SCGE to detect the DNA damage in fish blood cells and liver cells. It showed that this assay could be applied in the assessment of water pollution and aquatic mutagens.