生物膜水解-好氧循环系统处理活性艳蓝RB-19的微生物群落动态变化

为了研究生物膜水解-好氧循环系统处理蒽醌类染料活性艳蓝RB-19效果及其中微生物群落动态变化,利用基于16S rDNA的PCR-DGGE技术获得了微生物群落的DNA特征指纹图谱。结果表明,该系统能有效地降解活性艳蓝RB-19模拟废水,在RB-19浓度≤400 mg/L时,RB-19去除率维持在82%～96%之间,COD去除率维持在95%左右,但当RB-19浓度提高到500 mg/L时,RB-19去除率降低到58%,COD去除率降低到85%。DGGE分析表明,生物膜上的微生物群落结构随着RB-19浓度递增有显著变化,好氧、水解反应器内的细菌Shannon指数分别从1.32和1.20降低到1.11和1.19。UPGMA聚类分析和NMDS散点分析表明,水解、好氧反应器内的微生物并没有因为同处一个系统内而使得其菌群落结构产生明显的趋同倾向。系统内的多种优势菌群为兼性细菌,克隆测序的结果发现,在水解反应器存在一类具有很强还原能力的古细菌——Methanobacterium sp.MB4。

Microbial community dynamics in biofilm hydrolytic-aerobic recycling system treating Reactive Blue 19

In order to study the ability of the biofilm hydrolytic-aerobic recycling system(BHARS) in dealing with anthraquinone dye and reveal the succession of microbial community dynamics in this system,the genome DNA of biofilm was directly extracted.PCR-amplified 16S rDNA and denaturing gradient gel electrophoresis(DGGE) were applied to obtain the DNA fingerprint profile of the microbial communities.The results show that the system can reach the removal efficiency of color and COD at 82%~96% and 95% while the con...