剩余污泥超声提取液培养生物破乳菌Alcaligenes sp. S- XJ-1

将超声法预处理剩余污泥得到的提取液用于培养高效生物破乳菌Alcaligenes sp. S-XJ-1,以探索剩余污泥资源化利用的新途径.使用超声能量(ES)为443~56647kJ/kg TS的超声条件处理剩余污泥,获得的污泥提取液中氨氮、有机氮、总磷的浓度最高分别为171.94,142.20, 76.29mg/L,提取液中包含K、Ca、Mg、Fe等破乳菌生长必须的金属元素.将污泥提取液(ES为885~56647kJ/kg TS)作为培养基用于合成高效破乳菌Alcaligenes sp. S-XJ-1,产量较MMSM培养基可提高0.3%~68.3%,其中ES为56647kJ/kg TS时破乳菌产量最高,为2.03g/L.污泥提取液中的pH缓冲能力对菌体产量有重要影响,提取液的有机氮浓度可能是破乳菌产量提高的关键因素.污泥提取液培养的破乳菌破乳性能稳定保持在80.0%左右,菌体细胞表面疏水性与菌体C/N较MMSM培养基培养菌体无明显差异.说明以剩余污泥超声提取液作为培养基可以培养稳定高效的破乳菌Alcaligenes sp. S-XJ-1.

Cultivation of demulsifying bacteria Alcaligenes sp. S-XJ-1 using ultrasonic pretreated excess sludge

In order to find a new way of excess sludge utilization, sludge extract was used in the biosynthesis of demusifier by Alcaligenes sp. S-XJ-1. It was practical to cultivate highly efficient demulsifying strains in sludge extract. Sludge extract was obtained by the untrasound condition of 443~56647kJ/kg TS. In sludge culture medium, maximun concentration of ammonia nitrogen, organic nitrogen and phosphorus were 171.94,142.20 and 76.29mg/L. Sludge extract contained metallic elements such as K, Ca, Mg, Fe that were essential to the synthesis of S-XJ-1. After 7days cultivation, the biomass of S-XJ-1in sludge extract was 0.3%~68.3% higher than that in MMSM. Highest yield (2.03g/L) was obtained when specific energy was 56647KJ/kg TS. pH buffering capacity and concentration of organic nitrogen might have a great impact on the yield. The bio-demusifier produced with sludge extract had a quite stable demusifying ration of 80%, of which the CSH and C/N did not show much difference to that with MMSM.