低浓度五氯酚暴露对稀有鮈鲫体内SOD活性、GSH和HSP70含量的影响

为筛选检测低浓度五氯酚(PCP)对水生生物毒性效应较敏感的指标,将稀有鮈鲫(Gobiocypris rarus)暴露于浓度分别为0.5、1.5、5.0、15和50μg·L-1的PCP中2、7、14、21d,同时设乙醇对照组,检测稀有鮈鲫体内(整体匀浆)超氧化物歧化酶(SOD)活性、谷胱甘肽(GSH)、热激蛋白70(HSP70)含量等生化指标.结果表明,相同暴露时间下,随着PCP暴露浓度的升高,SOD活性均表现为先抑制后激活趋势,GSH和HSP70含量无明显变化;相同暴露浓度下,随着PCP暴露时间的延长,SOD活性均表现为先激活后抑制趋势,GSH和HSP70含量无明显变化.初步认定相对于GSH、HSP70含量,SOD活性的变化可以更有效地反映低浓度PCP对稀有鮈鲫的毒性效应.

Effects of Low Concentration of Pentachlorophenol Exposure on SOD Activity,GSH and HSP70 Content in Rare Minnow(Gobiocypris rarus)

In order to screen the sensitive index to detect the toxic effect of low concentration pentachlorophenol(PCP)on aquatic creature,SOD activity,GSH and HSP70 content were measured in whole homogenate of rare minnow(Gobiocypris rarus)which exposed to the PCP concentration with 0.5,1.5,5.0,15 and 50μg·L-1 for 2,7,14 and 21 days,and 0.01% ethanol served as negative control.Results showed that the SOD activity was induced at first and then inhibited with the increase of PCP concentrations at the same exposure time,while the GSH and HSP70 content showed no significant change compared with the control;the SOD activity was inhibited at first and then induced with the increase of exposure time at the same exposure concentrations,while the GSH and HSP70 content showed no significant change compared with the control.According to the preliminary analysis,the SOD activity can reflect the toxic effect of low concentration PCP on rare minnow more effectively compared with the GSH and HSP70 content.