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Localization of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and 2,4,6-trinitrotoluene (TNT) in poplar and switchgrass plants using phosphor imager autoradiography |
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| Authors: | Laura B Brentner Sachiyo T Mukherji Jerald L Schnoor |
| Institution: | a Chemical Engineering and Environmental Engineering Program, Yale University, New Haven, CT 06513, USA b Department of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA c Small Animal Imaging Core, The University of Iowa, Iowa City, IA 52242, USA d Department of Civil and Environmental Engineering, The University of Iowa, Iowa City, IA 52242, USA |
| Abstract: | Phosphor imager autoradiography is a technique for rapid, sensitive analysis of the localization of xenobiotics in plant tissues. Use of this technique is relatively new to research in the field of plant science, and the potential for enhancing visualization and understanding of plant uptake and transport of xenobiotics remains largely untapped. Phosphor imager autoradiography is used to investigate the uptake and translocation of the explosives 1,3,5-trinitro-1,3,5-triazine (RDX) and 2,4,6-trinitrotoluene within Populus deltoides × nigra DN34 (poplar) and Panicum vigratum Alamo (switchgrass). In both plant types, TNT and/or TNT-metabolites remain predominantly in root tissues while RDX and/or RDX-metabolites are readily translocated to leaf tissues. Phosphor imager autoradiography is further investigated for use in semi-quantitative analysis of uptake of TNT by switchgrass. |
| Keywords: | Phosphor imager Autoradiography 2 4 6-Trinitrotoluene (TNT) 1 3 5-Trinitro-1 3 5-triazine (RDX) Phytoremediation Nuclear waste Radioactive material |
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