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Lead deposited in the cell wall of Funaria hygrometrica protonemata is not stable - A remobilization can occur |
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| Authors: | Magdalena Krzes?owska Marta Lenartowska Henryk Bilski |
| Institution: | a Laboratory of General Botany, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland b Laboratory of Developmental Biology, Institute of General and Molecular Biology, Nicolaus Copernicus University, Gagarina 2, 87-100 Toruń, Poland c Laboratory of Electron and Confocal Microscopy, Adam Mickiewicz University, Umultowska street 89, 61-614 Poznań, Poland d Laboratory of Electron Microscopy, Nencki Institute of Experimental Biology, Pasteur Street 3, 02-093 Warszawa, Poland |
| Abstract: | The hypothesis that lead (Pb) can be uptake or remobilized from the cell wall (CW) by internalization withlow-esterified pectins (up to 40% - JIM5-P), was studied in tip-growing apical cell of Funaria hygrometrica protonemata. Treatment 4 h with 1 mM PbCl2 caused marked vesicular traffic intensification and the common internalization of JIM5-P from the CW. Lead bound to JIM5-P was internalized from the CW, together with this compound and entered the protoplast. It showed that Pb deposited in CW is not as safe for plant cell as previously believed.However, pulse-chase experiments (recovering 4 h and 24 h) indicated that CW and its thickenings can function as the final sequestration compartments. In Pb deposition sites, a callose layer occurred. It was localized from the protoplast site, next to Pb deposits separating sequestrated to CW and its thickenings Pb from plasma membrane almost certainly protecting the plant cell from its returning into the protoplast. |
| Keywords: | Pectin Tip growth Vesicle trafficking Pb Heavy metal |
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