Anaerobic biodegradation of cellulosic material: Batch experiments and modelling based on isotopic data and focusing on aceticlastic and non-aceticlastic methanogenesis |
| |
| Authors: | X Qu VA Vavilin L Mazéas M Lemunier C Duquennoi P-J He T Bouchez |
| |
| Institution: | 1. State Key Laboratory of Pollution Control and Resources Reuse, Key Laboratory of Yangtze River Water Environment, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China;2. Cemagref, UR-HBAN, Parc de Tourvoie, Antony cedex F-92163, France;3. Water Problems Institute, Russian Academy of Sciences, 3 Gubkina, Moscow 119333, Russian Federation;4. Suez-Env, CIRADE, 38 Avenue Jean Jaures, 78440 Gargenville, France;1. Cnr, Istituto di Ricerca Sulle Acque, Area della Ricerca di Roma 1, Monterotondo (Roma), Italy;2. Asja Ambiente Italia S.p.A., via Ivrea 70, Rivoli (TO), Italy;1. Department of Civil and Environmental Engineering, University of Port Harcourt Choba, P.M.B 5323 Rivers State, Nigeria;2. Department of Civil Engineering, University of Benin, P.M.B 1154 Benin City, Edo State, Nigeria;3. Department of Civil and Environmental Engineering, University of Surrey, Surrey GU2 7XH, United Kingdom;1. School of Civil Engineering, Hefei University of Technology, Hefei 230009, China;2. Institute of Water Treatment and Wastes Reutilization, School of Civil Engineering, Hefei University of Technology, Hefei 230009, China;1. Irstea, UR HBAN, 1 rue Pierre-Gilles de Gennes, 92761, Antony, France;2. Suez – CIRSEE, 38 rue du Président Wilson, 78230, Le Pecq, France |
| |
| Abstract: | Utilizing stable carbon isotope data to account for aceticlastic and non-aceticlastic pathways of methane generation, a model was created to describe laboratory batch anaerobic decomposition of cellulosic materials (office paper and cardboard). The total organic and inorganic carbon concentrations, methane production volume, and methane and CO2 partial pressure values were used for the model calibration and validation. According to the fluorescent in situ hybridization observations, three groups of methanogens including strictly hydrogenotrophic methanogens, strictly aceticlastic methanogens (Methanosaeta sp.) and Methanosarcina sp., consuming both acetate and H2/H2CO3 as well as acetate-oxidizing syntrophs, were considered. It was shown that temporary inhibition of aceticlastic methanogens by non-ionized volatile fatty acids or acidic pH was responsible for two-step methane production from office paper at 35 °C where during the first and second steps methane was generated mostly from H2/H2CO3 and acetate, respectively. Water saturated and unsaturated cases were tested. According to the model, at the intermediate moisture (150%), much lower methane production occurred because of full-time inhibition of aceticlastic methanogens. At the lowest moisture, methane production was very low because most likely hydrolysis was seriously inhibited. Simulations showed that during cardboard and office paper biodegradation at 55 °C, non-aceticlastic syntrophic oxidation by acetate-oxidizing syntrophs and hydrogenotrophic methanogens were the dominant methanogenic pathways. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
