| TTC-脱氢酶还原法测定铜绿微囊藻活性 |
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| 引用本文： | 梁文艳,王珂,阮清鸳,王金丽.TTC-脱氢酶还原法测定铜绿微囊藻活性[J].环境科学学报,2008,28(9):1745-1750. |
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| 作者姓名： | 梁文艳 王珂 阮清鸳 王金丽 |
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| 作者单位： | 北京林业大学环境科学与工程学院,省部共建森林培育与保护教育部重点实验室,北京100083 |
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| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划) |
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| 摘 要： | 利用氯化三苯基四氮唑(TTC)-脱氢酶还原法定量地测定了铜绿微囊藻细胞的活性,并对影响活性测定的因素进行了分析与研究.结果显示,TTC浓度大于0.6%时会抑制酶活性,最适TTC浓度为0.2%;脱氢酶还原反应在Tris-HCI缓冲液中能正常进行,而在磷酸盐缓冲液中受到抑制,最适pH值为7.5～8.0;培养时间和培养温度对TTC脱氧酶还原反应的影响较大,最适培养时间为8～16h,最适培养温度为30～35℃;反应不需加入非离子去垢剂,吐温20和曲拉通X-100对脱氢酶反应会产生较强抑制作用.TTC还原所生成的三苯基甲 (TPF)可采用50%乙醇提取.研究显示TTC脱氢酶还原法可以很好地进行藻细胞活性定量化测定.
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| 关 键 词： | 铜绿微囊藻 细胞活性 氯化三苯基四氮唑(TTC) 脱氢酶 |
| 收稿时间： | 8/9/2007 2:24:20 PM |
| 修稿时间： | 2007/11/18 0:00:00 |
Viability determination of Microcystis aeruginosa by TTC-dehydrogenase assay |
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| LIANG Wenyan,WANG Ke,RUAN Qingyuan and WANG Jinli.Viability determination of Microcystis aeruginosa by TTC-dehydrogenase assay[J].Acta Scientiae Circumstantiae,2008,28(9):1745-1750. |
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| Authors: | LIANG Wenyan WANG Ke RUAN Qingyuan and WANG Jinli |
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| Institution: | The Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Environmental Science & Engineering, Beijing Forestry University, Beijing 100083,The Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Environmental Science & Engineering, Beijing Forestry University, Beijing 100083,The Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Environmental Science & Engineering, Beijing Forestry University, Beijing 100083 and The Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Environmental Science & Engineering, Beijing Forestry University, Beijing 100083 |
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| Abstract: | |
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| Keywords: | Microcystis aeruginosa algae viability 2 3 5-tripheyl tetrazolium Chloride(TTC) dehydrogenase |
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