双酚A降解菌的分离鉴定及其降解特性

从好氧堆肥反应器的渗滤液中分离到1株降解内分泌干扰物双酚A的细菌B-16.经过对其形态特征、生理生化以及16S rDNA序列分析,该菌株初步鉴定为Achromobacter xylosoxidans.通过摇瓶实验考察了生长条件对双酚A降解的影响,得出该菌株降解双酚A的最佳条件是接种量为0.6%,初始pH值为7.0,温度为30℃.在该条件下,对菌株B-16对不同初始浓度双酚A的降解反应过程进行动力学分析,该降解反应在低浓度(3、5、10mg/L)时符合一级反应动力学特征,而在较高底物浓度(20 mg/L5、0 mg/L)下不能用一级反应动力学描述,双酚A的降解率在初始浓度为10 mg/L时达到最大值46.93%.

Isolation, Identification and Degradation Characteristics of Bisphenol A Degrading Strain

A bisphenol A(BPA) degrading strain B-16 was isolated from compost leachate of municipal solid waste. According to the morphological characteristics, physiological and biochemical, and sequence analysis of 16S rDNA, the strain was identified preliminarily as Achromobacter xylosoxidans. By tests in shaking flasks, the effects of the conditions of growth was studied, and it was determined that the optimum conditions were inoculum amount 0.6%, pH 7.0, and 30 degrees C. Under these conditions, when the initial concentrations were low (3, 5, 10 mg/L), the degradation reaction tallied with one order kinetic characteristic, while when in high initial concentrations (20, 50 mg/L), the degradation reaction can't be described by one order kinetic characteristic. When the initial concentration was 10 mg/L, the degradation rate was maximal, and it was 46.93%.