Population structure of the wreckfish Polyprion americanus determined with microsatellite genetic markers |
| |
Authors: | A O Ball G R Sedberry M S Zatcoff R W Chapman J L Carlin |
| |
Institution: | (1) South Carolina Department of Natural Resources, 217 Fort Johnson Road, P.O. Box 12559, Charleston, South Carolina 29422-2559, USA Fax: 001 843 762-5110 e-mail: balla@mrd.dnr.state.sc.us, US |
| |
Abstract: | We examined population structure in the wreckfish, Polyprion americanus, by assaying six microsatellite loci in 422 individuals collected throughout the geographic range. Eighteen hapuku, P. oxygeneios, were assayed at the same loci for use as an outgroup. Expected heterozygosities ranged from 0.49 to 0.88 and averaged 0.66.
Allele-frequency distributions at those loci indicated that samples from the eastern North Atlantic, western North Atlantic
and the Mediterranean were genetically similar, confirming the pattern seen in a previous analysis of mitochondrial DNA (mtDNA)
restriction fragment length polymorphisms (RFLPs). Both mtDNA and microsatellite studies differentiated northern and southern
wreckfish stocks. However, in contrast to the mtDNA studies, allelic variation at microsatellite loci clearly differentiated
wreckfish from two Southern Hemisphere locations, Brazil and the South Pacific. Far more genetic variation was observed at
microsatellite loci than with mtDNA RFLPs (haplotype diversity averaged 0.01), and we saw more evidence of population structure
with the microsatellite loci. The differentiation between southern and northern wreckfish supports the distribution records,
which indicate that wreckfish do not occur in the tropics. Temperature profiles and current patterns throughout the southern
oceans apparently also prevent significant gene flow between the South Pacific and Brazilian samples.
Received: 29 January 2000 / Accepted: 27 June 2000 |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|