TBT对大鼠肝脏ROS、抗氧化酶和解毒系统酶的影响

 研究了三丁基锡(TBT)对大鼠肝脏ROS、抗氧化酶和解毒系统酶的影响.SD大鼠随机分为6组,TBT染毒剂量各组分别为,0,0.1,0.3,1.0,3.0,10.0mg/kg(B·W),连续灌胃7d,第8d处死,取肝脏检测活性氧(ROS)水平、谷胱甘肽转硫酶(GST)活性、过氧化氢酶(CAT)活性、谷胱甘肽还原酶(GR)活性和谷胱甘肽(GSH)含量.结果表明,ROS随TBT浓度增加而升高,各浓度组均有显著性差异.在0.3,1.0mg/kg时,GST活性升高且具有显著性差异,在3.0,10.0mg/kg有下降趋势;GR活性也升高且具有显著性差异.CAT活性及GSH含量在各浓度组均无明显变化.研究结果提示,染毒各组ROS水平明显升高,而与ROS去除有直接关系的GSH水平和CAT活性没有发生相关的变化,因此TBT在代谢转化过程中产生的自由基不能及时被清除可能是导致ROS水平明显升高的重要原因.

Influence of TBT on ROS, antioxidant enzymes and detoxification system enzyme in rat liver

The influence of tributyltin chloride(TBT) on the ROS, antioxidant enzymes and the detoxification system in rat liver was studied. SD rats were divided into 6 groups randomly. The TBT exposure dosages of each group were 0, 0.1, 0.3, 1.0, 3.0 and 10.0mg/kg(B?W) respectively. Consecutive gavage for 7d, the liver of the animals sacrificed on the 8th day was used to detect the reactive oxygen species (ROS) level, the activities of glutathione-S-transferase (GST), catalase (CAT), glutathione reductase (GR) and content of glutathione (GSH). The ROS increased with the increase of TBT concentration and there was significant difference in all every exposure groups. The activities of GST increased at concentration of 0.3 and 1.0mg/kg with significant difference; and tended to decrease in the groups of concentration 3.0 and 10.0mg/kg differences. No obvious change of the activities of CAT and the content of GSH in each exposure group existed. Therefore the important cause inducing increase of the ROS level might be that the free radicals produced in the metabolism transformation process of TBT could not be removed in time.