In vitro effect of dimethoate on the activity of tryptophan pyrrolase in rat liver |
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| Authors: | A A Hassan S M el-Sewedy Y Minatogawa R Kido |
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| Institution: | Applied Medical Chemistry Department, Alexandria university, Egypt. |
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| Abstract: | Total and holo-enzyme activities of tryptophan 2,3-dioxygenase were measured in vitro in the presence and absence of the organophosphorous insecticide, dimethoate. Addition of dimethoate to the reaction mixture decreased the activities of both total and holo-forms. Total and holo-enzyme activities were decreased by 34% and 26%, respectively, by 1 mM dimethoate. On the other hand, 5 mM dimethoate resulted in 56% and 34% inhibition to total and holo-enzyme activities, respectively. Lineweaver-Burk plot of the total-enzyme activity at different tryptophan concentration in the presence of 2 mM dimethoate gave uncompetitive type of inhibition. |
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