Microbial community structure and function during abnormal curve development of substrate-induced respiration measurements |
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| Institution: | 1. Scientific Information Center for Resources and Environment, National Science Library — Lanzhou Branch, Chinese Academy of Sciences, 8 Middle Tianshui Road, Lanzhou 730000, China;2. University of Delaware, Department of Civil & Environmental Engineering, 354A Du Pont Hall, Newark, DE 19716, USA;3. Lanzhou University, School of Resources and Environment, 222 South Tianshui Road, Lanzhou 730000, China;1. Institute of Geology and Palaeontology, Faculty of Science, Charles University, Albertov 6, 128 43, Praha 2, Czech Republic;2. Institute of Geology of the Czech Academy of Sciences, Rozvojová 269, 165 00 Praha, 6 Lysolaje, Czech Republic;3. Earth Science Institute of the Slovak Academy of Sciences, Ďumbierska 1, 974 11 Banská Bystrica, Slovakia;4. Institute of Geological Sciences, Masaryk University, Faculty of Science, Kotlářská 2, 611 37 Brno, Czech Republic |
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| Abstract: | Soil respiration measurements are an established method to test the abundance, activity and vitality of the soil microorganisms. However, abnormal progressions of soil respiration curves impede a clear interpretation of the data. The aim of this study was to investigate the changes in the microbial structure during the formation of phenomena like double peaks and terraces by analysis of the PLFA composition (phospholipid fatty acid composition). Moreover, 13C labeled glucose was used as substrate; therefore it was possible to measure δ13C values both within the PLFA fraction as well as within the carbon dioxide evolved during respiration. As contaminants trinitrotoluene, cycloheximide, and hexadecane were used. The results showed that the appearance of double peaks was mainly related to the growth of fungi with the marker 18:2δ9,12 due to a toxic effect of trinitrotoluene and cycloheximide. In contrast, the phenomenon of terrace formation was related to the utilization of hexadecane as a carbon source mainly by bacteria. |
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