Experimentally elevated plasma levels of testosterone do not increase male reproductive success in blue tits

In temperate passerines, increased testosterone (T) levels during breeding mediate male aggressive and mating behaviour. If individual variability in T levels is reflected in behavioural differences during mating, males with higher T might gain higher reproductive success. This can be tested experimentally by elevating T levels. However, high exogenous T levels are known to have negative effects on male sperm production. This may reduce male fitness, particularly if sperm competition is intense. We experimentally elevated T levels in breeding blue tit males to investigate how T levels above the natural mean influence male reproductive success. Contrary to most—if not all—previous experimental manipulations of T levels in birds, we restricted the treatment with exogenous T to the time when females were fertile and T levels were naturally high in males. In blue tits, extra-pair paternity is an important component of male reproductive success, and its frequency is likely influenced by androgen-mediated behaviours such as mate attraction and aggression towards other males. Here we show that T-males were equally likely to become cuckolded and did not gain more extra-pair paternity than control males. Cuckolded T-males, however, lost more paternity than control males. We discuss the possibility that this is caused by negative effects of T treatment on sperm production.Communicated by M. Webster