鲤鱼对纳米二氧化钛的生物富集

建立了浓硫酸-硫酸铵酸化消解-ICP测定水样及鱼样中纳米二氧化钛的方法,并研究了纳米二氧化钛在鲤鱼(Cyprinus carpio)体内的富集.酸化消解-ICP测定方法对20.0 mg/L纳米二氧化钛水样,测定的相对标准偏差仅为4.53%.纳米二氧化钛在水样和鱼样中的加标回收率分别为94%～104%和90%～103%,可以精确测定环境样品中纳米二氧化钛浓度.纳米二氧化钛在鱼体内有较高的富集,暴露于3 mg/L、10 mg/L纳米二氧化钛悬浮液的鲤鱼,25 d时鱼体内的二氧化钛浓度分别达到2.1 mg/g和5.8 mg/g.达到平衡时,BCF分别为675.5和595.4.纳米二氧化钛在鱼鳃、内脏中有很大程度的富集,肌肉部分对纳米二氧化钛的富集最少.

Bioaccumulation of Titanium Dioxide Nanoparticles in Carp

Analysis method for TiO2 nanoparticles in water and fish samples was set up using ICP-AES after dissolved by solution of sulphuric acid and ammonium sulphate. Meanwhile bioaccumulation of TiO2 nanoparticles was assessed by tests exposing carp (Cyprinus carpio)to TiO2 nanoparticles suspensions. The relative standard deviation (RSD) for water sample of 20.0 mg/L TiO2 is 4.53%, and the recoveries of TiO2 nanoparticles in water and fish samples are raging from 94% - 104% and 90% - 103%, which could ensure accurate measurement of TiO2 nanoparticles. TiO2 nanoparticles have a significant bioaccumulation by the carp, and TiO2 nanoparticles concentrations in carp exposed to 3 mg/L and 10 mg/L TiO2 nanoparticles suspensions for 25 days are 2.1 mg/g and 5.8 mg/g respectively, and the BCF values at equilibrium are 675.5 and 595.4 respectively. Significant As and TiO2 accumulation occurs in viscus and gills of fish, while bioaccumulation of TiO2 nanoparticles in muscle is relative small.