Cu2+和Cu-EDTA对鲫鱼脑组织应激蛋白HSP70诱导的影响

以鲫鱼(Carassius auratus)作为实验对象,经过40d不同浓度铜(Cu²⁺)及其配合物(Cu-EDTA)的暴露后,运用SDS-PAGE和Western Blotting方法检测鱼脑组织内应激蛋白HSP70的诱导表达情况.结果表明:在实验浓度下,与对照组相比,Cu²⁺和Cu-EDTA对鱼脑内HSP70有显著的诱导(p<0.05),而将Cu²⁺和Cu-EDTA实验组相对比,络合剂EDTA一定程度上改变了Cu的生物可利用性,从而降低了其毒性.实验还发现,Cu²⁺浓度在国家渔业水质标准0.01mg/L下时,鱼脑组织内HSP70已经有明显的表达,说明运用分子生物学指标要比传统的环境监测指标更敏感,具有对污染物早期预警的作用.

Effects of Copper (Cu2+) and Cu-EDTA Complex on the Induction of HSP70 in the Fish Brain

The effects of copper (Cu²⁺) and Cu-EDTA complex on the induction of HSP70 in the fish brain were studied.After the fish (Carassius auratus) were exposed to the experimental concentrations for forty days , the SDS-PAGE and Western Blotting techniques were used to detect the expression level of HSP70 in the fish brain.The results showed that Cu²⁺ and Cu-EDTA complex can induce the expression of HSP70 significantly.Comparing with the test groups exposed to Cu²⁺,the Cu-EDTA complex had changed the bioavailability and decreased the toxicity of the heavy metal.It was found that the fish exposed to the concentration of Cu²⁺ which below the national fishery water quality standard of 0.01mg/L , also had a significant increase in the expression level of HSP70. Therefore, HSP70 as an environmental biomarker can be a more sensitive and rapid indicator than conventional end points in the aquatic ecosystem.