| 澜沧江流域浮游细菌群落结构特征及驱动因子分析 |
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| 引用本文： | 程豹,望雪,徐雅倩,杨正健,刘德富,马骏.澜沧江流域浮游细菌群落结构特征及驱动因子分析[J].环境科学,2018,39(8):3649-3659. |
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| 作者姓名： | 程豹 望雪 徐雅倩 杨正健 刘德富 马骏 |
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| 作者单位： | 湖北工业大学土木建筑与环境学院河湖生态修复与藻类利用湖北省重点实验室 |
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| 基金项目： | 国家自然科学基金项目（91647207，51509086）；国家重点研究计划项目（2016YFC0402204，2016YFC0401702） |
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| 摘 要： | 为探究澜沧江流域的浮游细菌群落结构特征及驱动因子,应用16S rRNA高通量测序技术,分析了2017年2月澜沧江流域浮游细菌群落结构特征,并采用Pearson相关性分析(Pearson correlation analysis)和冗余分析(RDA)识别了澜沧江自然河道段和水库段浮游细菌群落结构变化的关键环境因子.结果表明,自然河道段ACE指数和Shannon指数均高于水库段,造成自然河道段和水库段浮游细菌多样性变化的主要环境因子为水温(WT)、溶解氧(DO)、浊度(Tur)、高锰酸盐指数(permanganate index)、p H和总氮(TN).对16S rRNA V3和V4测序,得到用于物种分类的OTU数共26772,涵盖了浮游细菌群落共45门,965属.菌群分类发现,变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)和放线菌门(Actinobacteria)为优势门,其中变形菌门(Proteobacteria)含量相对丰富,占细菌群落的36%~94%.澜沧江流域变形菌门(Proteobacteria)主要包括α-变形菌纲(α-Proteobacteria)、β-变形菌纲(β-Proteobacteria)和γ-变形菌纲(γ-Proteobacteria),分别占变形菌门(Proteobacteria)的比例为0.39%~21.56%、0.39%~55.80%和31.09%~99.18%.澜沧江水体浮游细菌群落空间差异明显,影响浮游细菌群落结构变化的环境因子主要为WT、高锰酸盐指数、Tur、DO和TN.自然河道段和水库段影响浮游细菌群落结构的环境因子不同,DO和Tur是影响自然河道段浮游细菌群落结构的关键环境因子,而水库段浮游细菌群落结构主要受WT、高锰酸盐指数和TN的影响.
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| 关 键 词： | 澜沧江 高通量测序 细菌多样性 群落结构 环境因子 |
| 收稿时间： | 2017/11/1 0:00:00 |
| 修稿时间： | 2018/2/8 0:00:00 |
Bacterioplankton Community Structure in the Lancang River Basin and the Analysis of Its Driving Environmental Factors |
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| CHENG Bao,WANG Xue,XU Ya-qian,YANG Zheng-jian,LIU De-fu and MA Jun.Bacterioplankton Community Structure in the Lancang River Basin and the Analysis of Its Driving Environmental Factors[J].Chinese Journal of Environmental Science,2018,39(8):3649-3659. |
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| Authors: | CHENG Bao WANG Xue XU Ya-qian YANG Zheng-jian LIU De-fu and MA Jun |
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| Institution: | Hubei Key Laboratory of Ecological Restoration of River-lakes and Algal Utilization, College of Civil Construction and Environment, Hubei University of Technology, Wuhan 430068, China,Hubei Key Laboratory of Ecological Restoration of River-lakes and Algal Utilization, College of Civil Construction and Environment, Hubei University of Technology, Wuhan 430068, China,Hubei Key Laboratory of Ecological Restoration of River-lakes and Algal Utilization, College of Civil Construction and Environment, Hubei University of Technology, Wuhan 430068, China,Hubei Key Laboratory of Ecological Restoration of River-lakes and Algal Utilization, College of Civil Construction and Environment, Hubei University of Technology, Wuhan 430068, China,Hubei Key Laboratory of Ecological Restoration of River-lakes and Algal Utilization, College of Civil Construction and Environment, Hubei University of Technology, Wuhan 430068, China and Hubei Key Laboratory of Ecological Restoration of River-lakes and Algal Utilization, College of Civil Construction and Environment, Hubei University of Technology, Wuhan 430068, China |
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| Abstract: | |
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| Keywords: | Lancang River high-throughput sequencing bacterial diversity community structure environmental factors |
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